5. The Effects of Ocean Acidification in Gastropod Shell Development

Keisuke Shimizu (1), Katsunori Kimoto (2)

1 JAMSTEC, Yokosuka, Kanagawa, 237-0061, JAPAN
2 JAMSTEC, Yokosuka, Kanagawa, 237-0061, JAPAN

Background
The Molluscs constitutes one of the most diverse animal phyla, and they have evolved calcified exoskeletons called “shell”. Current high levels of CO2 leads to ocean acidification, and it affects for molluscan shell development. In fact, many previous studies have reported their phenotypic effects by ocean acidification, but the genetic response remains meagre. Thus we sought to understand not only the phenotypic effect but also the effect of gene expression pattern in high pCO2 condition using marine limpet Nipponacmea fuscoviridis.

Methods
After the artificial fertilization, embryos were cultured in different pCO2 conditions, and we checked the effects in early development.

Findings
In high pCO2 condition, we observed phenotypic and genetic effects in trochophore and veliger larvae stages.

Conclusions
Our results provide new insight of the effects for calcifying organisms in the feature high pCO2 marine condition.