Metamorphic and proteomic responses of fast-growing and slow-growing larval cohorts of a Pacific oyster to ocean acidification and warming

Chair: Jessica Ericson

Ko Wai Kuen Ginger (1), Dineshram Ramadoss (1), Havenhand Jon (2), Thiyagarajan V (1)*

1 The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong SAR.
2 Department of Biological & Environmental Sciences, Tjärnö, University of Gothenburg, Sweden

Ocean acidification and warming have previously been shown to significantly affect the physiological performance of oyster larvae; however, those studies were mainly focus on the effect of fast growing cohort that represented less than 20% of the overall population as marine invertebrate larval development rate is known to be heterogeneous. Sweeping statements about the specie’s response to environmental changes would then be easily made. In this study, comparative larval physiology (metamorphic success) and molecular responses (iTRAQ protein analysis) of both fast and slow growing cohort of Pacific oyster larvae (Crassostrea gigas) to chronic environmental stresses of temperature (24 and 30°C) and pH (pH 8.1 and pH 7.4) are investigated in a >2-week laboratory study. Recruitment failure and stress-related proteins expression were observed only in slow growing larvae of Pacific oyster, suggesting that the effect of slow growing larvae is also important to be considered. This study therefore is important for understanding the mechanisms of both fast and slow growing larvae of Pacific oyster respond to changing environments and projecting the effects of global climate change on oyster aquaculture in China.